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Depositors
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New England Biolabs, Inc., PA Rees, New England Biolabs, Inc.
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Vector Information
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Size (kb): 5.1999998092651370 Vector: pBIIHI.2 (plasmid) Promoters: Promoter str Construction: pNO1523 Marker(s):ampR Construct size (kb): 5.199999809265137 Features: insert detection: rpsL+
marker(s): ampR
promoter: str
replicon: pMB1
terminator: none
enhancer: none
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Applications
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vector permitting positive selection for inserts
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; BglII--5.2; HindIII--5.2; PstI--3.6, 1.6. Permits positive selection for inserts, Based on the dominance of the wild-type rpsL (ribosomal protein S12) gene in the plasmid. The SmaI and one HpaI site are in this gene. Derived from pNO1523 by inserting a BglII linker at the EcoRI site and a HpaI linker at the PvuII site. The order of the major features in this plasmid is: EcoRI - BglII - EcoRI - ClaI - HindIII - BamHI - rpsL - ori - bla.
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References
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Rees PA, Brenner JS II. Method for producing the HincII restriction endonuclease and methylase. US Patent 5,015,581 dated May 14 1991
Waite-Rees PA, et al. Characterization and expression of the Escherichia coli Mrr restriction system. J. Bacteriol. 173: 5207-5219, 1991. PubMed: 1650347
Phyllis A Rees, personal communication
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