pBIIHI.2 [NEB 533] (ATCC® 67902)

Applications: vector permitting positive selection for inserts  /  Depositors: New England Biolabs, Inc., PA Rees, New England Biolabs, Inc.

Permits and Restrictions

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Designations pBIIHI.2 [NEB 533]
Depositors New England Biolabs, Inc., PA Rees, New England Biolabs, Inc.
Biosafety Level 1
Vector Information
Size (kb): 5.1999998092651370
Vector: pBIIHI.2 (plasmid)
Promoters: Promoter str
Construction: pNO1523
Marker(s):ampR
Construct size (kb): 5.199999809265137
Features: insert detection: rpsL+
marker(s): ampR
promoter: str
replicon: pMB1
terminator: none
enhancer: none
Applications
vector permitting positive selection for inserts
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; BglII--5.2; HindIII--5.2; PstI--3.6, 1.6.
Permits positive selection for inserts,
Based on the dominance of the wild-type rpsL (ribosomal protein S12) gene in the plasmid. The SmaI and one HpaI site are in this gene.
Derived from pNO1523 by inserting a BglII linker at the EcoRI site and a HpaI linker at the PvuII site.
The order of the major features in this plasmid is: EcoRI - BglII - EcoRI - ClaI - HindIII - BamHI - rpsL - ori - bla.
Media ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Growth Conditions
Temperature: 37.0°C
References

Rees PA, Brenner JS II. Method for producing the HincII restriction endonuclease and methylase. US Patent 5,015,581 dated May 14 1991

Waite-Rees PA, et al. Characterization and expression of the Escherichia coli Mrr restriction system. J. Bacteriol. 173: 5207-5219, 1991. PubMed: 1650347

Phyllis A Rees, personal communication

Shipped freeze-dried
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