#595 [S-595] (ATCC® 67755)

Applications: contains sequence interleukin 1, beta  /  Depositors: Otsuka Pharmaceuticals Co., Ltd., PM Arthur, Otsuka Pharmaceuticals Co., Ltd.

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Designations #595 [S-595]
Depositors Otsuka Pharmaceuticals Co., Ltd., PM Arthur, Otsuka Pharmaceuticals Co., Ltd.
Biosafety Level 1
Vector Information
Size (kb): 4.5560002326965330
Vector: #595 (plasmid)
Promoters: Promoter for expression lambda PR
Marker(s):ampR
Construct size (kb): 4.556000232696533
Features: marker(s): ampR
promoter for expression: lambda PR
replicon: pMB1
repressor gene: cI857
restriction site: BamHI
restriction site: ClaI
terminator: lambda 4s
coding sequence: IL-1, mature peptide
Applications
contains sequence interleukin 1, beta
Comments
A putative RBS can be cloned into the ClaI site using a double stranded oligonucleotide having a GC overhang at the 3' end.
The vector allows testing of various oligonucleotide sequences as suitable ribosome binding sites (RBS) for expression of the gene in prokaryotic cells. The plasmid construct lacks a 5' leader sequence and RBS.
The optimal RBS may vary from protein to protein. To facilitate testing other proteins, the insert coding sequence can be replaced by other foreign gene sequences, using ClaI and BamHI. The foreign gene must provide its own ATG initiation codon.
Recommendation for verification: BamHI+ClaI, BglI, PstI.
Media Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Growth Conditions
Temperature: 30.0°C
References

Arthur PM, Duckworth BC. Gene regulation cassettes, expression vectors containing the same and microorganisms transformed with the same. US Patent 5,427,924 dated Jun 27 1995

Product Sheet
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Product Sheet