pE5LVP0 (ATCC® 67525)

Applications: contains sequence genome, partialvector permitting RNA synthesis in vitro  /  Depositors: Wisconsin Alumni Res. Fndn., G Parks, Wisconsin Alumni Res. Fndn.

Permits and Restrictions

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Designations pE5LVP0
Depositors Wisconsin Alumni Res. Fndn., G Parks, Wisconsin Alumni Res. Fndn.
Biosafety Level 1
Vector Information
Size (kb): 4.8000001907348630
Vector: pE5LVP0 (plasmid)
Promoters: Promoter T7
Construction: pSPT18, EMC
Marker(s):ampR
Construct size (kb): 4.800000190734863
Features: marker(s): ampR
promoter: SP6, T7
replicon: pMB1
enhancer: EMC
Applications
contains sequence genome, partial
vector permitting RNA synthesis in vitro
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; HindIII--3.28, 1.54; XbaI--4.8.
Permits increased expression for in vitro transcription translation systems by providing a translational enhancer downstream of the T7 promoter.
Digestion of the plasmid with XbaI plus BalI generates a fragment (approximately 4 kb) that has no EMC coding regions, but retains the translational enhancer. This can be used for cloning.
If a potential insert contains transcription terminators, then the BalI site can be used for cloning.
For in vitro transcription, the plasmid should be linearized with XbaI.
Contains DNA corresponding to nt 260 through 2004 of encephalomyocarditis virus RNA, including the 5' non-coding region and coding regions for L, 1A, and IB.
The order of the major features in this plasmid is: T7 promoter - 5' non-coding region of EMC - BalI - partial coding regions of EMC - XbaI - MCS - pSPT18.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Palmenberg AC, et al. Translation enhancer. US Patent 4,937,190 dated Jun 26 1990

Ann C Palmenberg, personal communication

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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