pEG5005 (ATCC® 67463)

Applications: cloning vectorintegrating vector  /  Depositors: OmniGene, Inc., P Mahler, OmniGene, Inc.

Permits and Restrictions

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Designations pEG5005
Depositors OmniGene, Inc., P Mahler, OmniGene, Inc.
Biosafety Level 1
Vector Information
Size (kb): 10.1999998092651400
Vector: pEG5005 (plasmid)
Construction: Mu d5005, pUC9 delta(lac)
Marker(s):ampR,kanR
Construct size (kb): 10.19999980926514
Features: marker(s): ampR
marker(s): kanR
replicon: pMB1
repressor gene: Mu cts62
MCS: PstI...BamHI
restriction site: ClaI
restriction site: SstII
coding sequence: A
coding sequence: B
Applications
cloning vector
integrating vector
Comments
Restriction digests of the clone give the following sizes (kb): ClaI--10.2,; EcoRI--8.8, 1.5; HindIII--5.2, 5.0.
To maintain the plasmid, grow at 30C with ampicillin and kanamycin selection.
Plasmid containing the mini-bacteriophage Mu replicon Mu d5005.
Media ATCC® Medium 1948: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 20 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C
References

Balakrishnan R, et al. A gene cassette for adapting Escherichia coli strains as hosts for att-Int-mediated reqrrangement and pL expression vectors. Gene 138: 101-104, 1994. PubMed: 8125284

Groisman EA, Casadaban MJ. Mini-Mu bacteriophage with plasmid replicons for in vivo cloning and lac gene fusing. J. Bacteriol. 168: 357-364, 1986. PubMed: 3020001

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet