pCLL4 (ATCC® 67447)

Applications: shuttle vector  /  Depositors: American Cyanamid Co., JS Feitelson, American Cyanamid Co.

Permits and Restrictions

View Permits

Designations pCLL4
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Depositors American Cyanamid Co., JS Feitelson, American Cyanamid Co.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 10.36
Vector: pCLL4 (plasmid)
Construction: pAkij1, pIJ39
Marker(s):ampR,tsrR
Construct size (kb): 10.36
Features: marker(s): ampR
marker(s): tsrR
replicon: pAkij1
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--9.0, 2.1; PstI--10.5; KpnI--10.5.
Cloning into the PstI site inactivates the ampicillin marker used for selection in Escherichia coli.
The pAkij1 ori may be useful in other taxa such as Bacillus, Staphylococcus and other Actinomycetes, including Streptosporangium, Actinoplanes, Nocardia, and Micromonospora.
Recommendation for verification: HindIII--8.37, 1.99; PstI--10.36; KpnI--9.47, 0.89
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Feitelson JS. Multifunctional plasmid vectors from Actinomadura and Escherichia coli. US Patent 5,002,891 dated Mar 26 1991

Feitelson JS, et al. Genetic and biochemical characterization of the red gene cluster of streptomyces coelicolor A3(2). J. Gen. Microbiol. 131: 2431-2441, 1985. PubMed: 2999302

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid

Product Sheet
Product Sheet
Product Sheet
Attachments Map of pCLL4, ATCC 67447