pYAC2 (ATCC® 67380)

Applications: YL-type (YAC) shuttle vectorshuttle vectorvector for constructing genomic libraries  /  Depositors: MV Olson

Permits and Restrictions

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Designations pYAC2
Depositors MV Olson
Biosafety Level 1
Vector Information
Size (kb): 11.1000003814697300
Vector: pYAC2 (YAC)
Marker(s):TRP1,URA3,ampR,HIS3,SUP4
Construct size (kb): 11.10000038146973
Features: marker(s): ampR, HIS3, SUP4, TRP1, URA3
replicon: pMB1, ARS1
centromere: CEN4
Applications
YL-type (YAC) shuttle vector
shuttle vector
vector for constructing genomic libraries
Comments
A yeast artificial chromosome vector for cloning blunt-ended fragments.
This yeast artificial chromosome contains ARS1, CEN4 and Tetrahymena telomeric elements.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Burke DT, et al. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812, 1987. PubMed: 3033825

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet