pSM214 (ATCC® 67320)

Applications: expression vectorshuttle vector  /  Depositors: G Grandi

Permits and Restrictions

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Designations pSM214
Depositors G Grandi
Biosafety Level 1
Vector Information
Size (kb): 7.4000000953674320
Vector: pSM214 (plasmid)
Promoters: Promoter T5
Construction: pUB110, pBR322, bacteriophage lambda
Marker(s):ampR,cmlR,kanR
Construct size (kb): 7.400000095367432
Features: marker(s): ampR, cmlR, kanR
promoter: T5
replicon: pUB110, pMB1
terminator: lambda to
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.5; HindIII--7.5; SstI--7.5; XbaI--5.8, 1.8.
Media ATCC Medium 1065 (see below) plus kanamycin (5 mcg/ml) and chloramphenicol (5 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 30.0°C
Shipping Information Distributed: Frozen
Product Sheet
Product Sheet
Product Sheet