pBC102-K22 (ATCC® 67255)

Applications: YE-type (episomal) shuttle vectorexpression vectorvector permitting construction of fusion proteinsvector with sequences for protein export  /  Depositors: Immunex Corporation, SG Hallquist, Immunex Corporation

Permits and Restrictions

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Designations pBC102-K22
Depositors Immunex Corporation, SG Hallquist, Immunex Corporation
Biosafety Level 1
Vector Information
Size (kb): 9.3999996185302730
Vector: pBC102-K22 (plasmid)
Promoters: Promoter ADH2
Construction: pBR322
Marker(s):TRP1,ampR
Construct size (kb): 9.399999618530273
Features: marker(s): ampR, TRP1
promoter: ADH2
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
expression vector
vector permitting construction of fusion proteins
vector with sequences for protein export
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.4; BamHI--uncut; HindIII--6.5, 2.1, 0.8; PstI--5.8, 1.9, 1.7.
Shuttle expression vector utilizing sequences encoding the alpha factor leader peptide to allow secretion of recombinant proteins.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Curtis BM, et al. Fusion proteins comprising GM-CSF and IL-3. US Patent 5,073,627 dated Dec 17 1991

Curtis BM, et al. DNA sequences encoding fusion proteins comprising GM-CSF and IL-3. US Patent 5,108,910 dated Apr 28 1992

Shipped frozen
Shipping Information Distributed: freeze-dried
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