pGS1251 (ATCC® 53399)

Depositors: Plant Genetic Systems, R Meeusen, Plant Genetic Systems

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Designations pGS1251
Depositors Plant Genetic Systems, R Meeusen, Plant Genetic Systems
Biosafety Level 1
Host
Distribution host: Agrobacterium tumefaciens C58C1
Vector Information
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pJRtac99
Intact vector size: 1.392
Type of vector: plasmid
Cloning sites: ScaI PstI SalI AccI AvaI SmaI EarI
Polylinker sites: ScaI PstI SalI AccI AvaI SmaI EarI BglII
Construction: pGV451, tac, Tn9 cat
Host range: Escherichia coli
Features (with orientation and position when available):
promoter: tac, ->, 20-115
marker(s): cmlR, ->, 121-780
MCS: , 752-798
replicon: HyRep2, 912-1392
Cross references: DNA Seq. Acc.: X69551
Media ATCC Medium 1065 (see below) plus streptomycin (100 mcg/ml) plus thymidine (50 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 37.0°C
References

De Greve HM, et al. Transformation vectors allowing expression of Bacillus thuringiensis endotoxins in plants. US Patent 5,254,799 dated Oct 19 1993

Shipping Information Distributed: freeze-dried
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