pMW1 plasmid in E. coli (ATCC® 39933)

Applications: contains sequence repressor protein cI857 mutation  /  Depositors: Amgen, Inc., F Lin, Amgen, Inc.

Permits and Restrictions

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Designations pMW1 plasmid in E. coli
Depositors Amgen, Inc., F Lin, Amgen, Inc.
Biosafety Level 1
Host
Distribution host: Escherichia coli JM103
Vector Information
Size (kb): 12.00
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): kanR, tetR
Cross references:
Vector: pMW1 (plasmid)
Marker(s):kanR,tetR
Construct size (kb): 12.0
Features: marker(s): kanR, tetR
replicon: pSH1
repressor gene: cI857
Applications
contains sequence repressor protein cI857 mutation
Comments
Temperature sensitive, grow at 28C.
A plasmid to provide a temperature sensitive lambda cI857 repressor for regulated expression of cloned sequences from a lambda promoter.
Has a pSHI replicon, compatible with colE1 vectors.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 28.0°C
References

Lin FK. DNA sequences encoding erythropoietin. US Patent 4,703,008 dated Oct 27 1987

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Product sheet