pMW1 plasmid in E. coli (ATCC® 39933)

Applications: contains sequence repressor protein cI857 mutation  /  Depositors: Amgen, Inc., F Lin, Amgen, Inc.

Permits and Restrictions

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Designations pMW1 plasmid in E. coli
U.S. Patent
Depositors Amgen, Inc., F Lin, Amgen, Inc.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli JM103
Vector Information
Size (kb): 12.00
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): kanR, tetR
Cross references:
Vector: pMW1 (plasmid)
Construct size (kb): 12.0
Features: marker(s): kanR, tetR
replicon: pSH1
repressor gene: cI857
contains sequence repressor protein cI857 mutation
Temperature sensitive, grow at 28C.
A plasmid to provide a temperature sensitive lambda cI857 repressor for regulated expression of cloned sequences from a lambda promoter.
Has a pSHI replicon, compatible with colE1 vectors.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 28.0°C

Lin FK. DNA sequences encoding erythropoietin. US Patent 4,703,008 dated Oct 27 1987

Shipped freeze-dried