pCFM526 (ATCC® 39932)

Applications: expression vector  /  Depositors: Amgen, Inc., F Lin, Amgen, Inc.

Permits and Restrictions

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Designations pCFM526
Depositors Amgen, Inc., F Lin, Amgen, Inc.
Biosafety Level 1
Host
Distribution host: Escherichia coli AM7/pMW1
Vector Information
Size (kb): 4.6500000953674320
Vector: pCFM526 (plasmid)
Promoters: Promoter lambda PL
Construction: pCFM414
Marker(s):ampR,kanR
Construct size (kb): 4.650000095367432
Features: marker(s): ampR, kanR
promoter: lambda PL
replicon: R1
terminator: lambda Toop
Applications
expression vector
Comments
Temperature sensitive. Grow at 28C.
Ampicillin is the selection marker for pCFM526, kanamycin for pMW1.
This is an expression plasmid containing a lambdaPL promoter, a ribosome binding site, a multiple cloning sequence, and a lambda Toop terminator.
This vector contains the copA region from NR1, the copB region from Beu1, and the repA and ori sequences of NR1. There is a G/C to A/T mutation at bp 598 in the copB coding region.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 28.0°C
References

Lin FK. DNA sequences encoding erythropoietin. US Patent 4,703,008 dated Oct 27 1987

Morris CF. DNA plasmids. US Patent 4,710,473 dated Dec 1 1987

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet