pRY255A (ATCC® 39822)

Applications: YI-type (integrating) shuttle vectorvector permitting positive selection for integrationvector permitting visual detection of recombinants  /  Depositors: RR Yocum

Designations pRY255A
Depositors RR Yocum
Biosafety Level 1
Host
Distribution host: Escherichia coli YMC9
Vector Information
Size (kb): 18.5000000000000000
Vector: pRY255A (plasmid)
Promoters: Promoter CYC1
Construction: pRY253
Marker(s):ampR G418R
Construct size (kb): 18.5
Features: marker(s): G418R
marker(s): ampR
other: HO
promoter: CYC1
replicon: pMB1
coding sequence: GAL1/lacZ fusion
Applications
YI-type (integrating) shuttle vector
vector permitting positive selection for integration
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--11.5, 7.0; KpnI--18.5; SalI--11.5, 7.0; SstI--13.0, 5.5; StuI--18.5.
A YI-type (yeast integrating, HO gene) shuttle vector that confers G418 resistance under control of the CYC1 promoter. Expresses a GAL1/lacZ fusion product allowing color selection.
Constructed from pRY253 (ATCC 39688) by replacing a SalI fragment with a XhoI/SalI fragment encoding the GAL1/lacZ fusion.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yocum RR, et al. Industrial yeast comprising an integrated glucoamylase gene. US Patent 5,422,267 dated Jun 6 1995

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet