pDG141 [CMCC 1966] (ATCC® 39588)

Applications: expression vector  /  Depositors: Cetus Corp., A Belt, Cetus Corp.

Permits and Restrictions

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Designations pDG141 [CMCC 1966]
Depositors Cetus Corp., A Belt, Cetus Corp.
Biosafety Level 1
Host
Distribution host: Escherichia coli
Vector Information
Size (kb): 2.5000000000000000
Vector: pDG141 (plasmid)
Promoters: Promoter trp
Construction: pBR322
Marker(s):ampR
Construct size (kb): 2.5
Features: marker(s): ampR
promoter: trp
replicon: pMB1
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI--2.5; EcoRI--2.5.
This is an expression vector with a tryptophan promoter-operator and ribosome binding site operably linked with an ATG start codon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gelfand DH, et al. Purified thermostable enzyme. US Patent 4,889,818 dated Dec 26 1989

Gelfand DH, Lawyer FC. Portable temperature-sensitive control cassette. US Patent 4,711,845 dated Dec 8 1987

Gelfand DH, et al. Universal dominant selectable marker cassette. US Patent 4,784,949 dated Nov 15 1988

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet