p219 (ATCC® 39550)

Applications: YE-type (episomal) shuttle vectorexpression vectorshuttle vector  /  Depositors: Immunex Corporation

Permits and Restrictions

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Designations p219
Depositors Immunex Corporation
Biosafety Level 1
Host
Distribution host: Escherichia coli
Vector Information
Size (kb): 8.8000001907348630
Vector: p219 (plasmid)
Promoters: Promoter unspecified yeast
Marker(s):TRP1,ampR
Construct size (kb): 8.800000190734863
Features: marker(s): ampR, TRP1
promoter: unspecified yeast
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
expression vector
shuttle vector
Comments
An EcoRI/HindIII double digest will give a 7.4 kb fragment of the vector retaining all the vector functions. The EcoRI site is just downstream of the yeast promoter.
Restriction digests of the clone give the following sizes (kb): EcoRI--8.8; PstI--7.2, 1.6; HindIII--6.6, 2.2; XbaI--6.85, 1.95.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Hopp TP, et al. Synthesis of protein with an identification peptide. US Patent 4,703,004 dated Oct 27 1987

Hopp TP, et al. Synthesis of protein with an identification peptide, and hybrid polypeptide incorporating same. US Patent 4,782,137 dated Nov 1 1988

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet