pMTchi5/4269C (ATCC® 39394)

Applications: YE-type (episomal) shuttle vectorexpression vectorshuttle vector  /  Depositors: Sandoz Crop Protection, PA Okubara, Sandoz Crop Protection

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Designations pMTchi5/4269C
Depositors Sandoz Crop Protection, PA Okubara, Sandoz Crop Protection
Biosafety Level 1
Host
Distribution host: Escherichia coli LE392
Vector Information
Size (kb): 10.8000001907348600
Vector: pMTchi5/4269C (plasmid)
Promoters: Promoter Ti
Construction: MTX5, Ti promoter
Marker(s):LEU2,ampR,DHFR
Construct size (kb): 10.80000019073486
Features: marker(s): ampR, DHFR, LEU2
promoter: Ti
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
expression vector
shuttle vector
Comments
This vector contains the following sites separated by (kb): BamHI- 0.15- PvuII- 0.33- BamHI- 9.2- PvuII- 1.6- BamHI.
Restriction digests of the clone give the following sizes (kb): HindIII--11.3; PvuII--9.6, 2.0; BamHI--11.3.
An episomal Saccharomyces cerevisiae/Escherichia coli shuttle vector.
A 480 bp HindIII/Sau3AI fragment from pTiA6 was isolated, the Sau3AI site changed to BamHI and the ends filled. BamHI linkers were added and it was cloned into the BamHI site of MTX5.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Okubara PA, et al. Hybrid plasmid with marker. US Patent 4,670,399 dated Jun 2 1987

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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