pHV33 (ATCC® 39217)

Applications: shuttle vector  /  Depositors: Miles Laboratories, Inc., KL Wollweber, Miles Laboratories, Inc.

Permits and Restrictions

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Designations pHV33
Depositors Miles Laboratories, Inc., KL Wollweber, Miles Laboratories, Inc.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli RR1; K-12 RR1
Vector Information
Size (kb): 6.9000000953674320
Vector: pHV33 (plasmid)
Marker(s):ampR,cmlR,tetR
Construct size (kb): 6.900000095367432
Features: marker(s): ampR, cmlR, tetR
replicon: pMB1
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.9; HindIII--4.65, 3.05.
This is an unstable, tetracycline-resistant mutant of pHV14.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Primrose SB, Ehrlich SD. Isolation of plamid deletion mutants and study of their instability. Plasmid 6: 193-201, 1981. PubMed: 7029580

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet