pHV33 (ATCC® 39217)

Applications: shuttle vector  /  Depositors: Miles Laboratories, Inc., KL Wollweber, Miles Laboratories, Inc.

Permits and Restrictions

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Designations pHV33
Depositors Miles Laboratories, Inc., KL Wollweber, Miles Laboratories, Inc.
Biosafety Level 1
Host
Distribution host: Escherichia coli RR1; K-12 RR1
Vector Information
Size (kb): 6.9000000953674320
Vector: pHV33 (plasmid)
Marker(s):ampR,cmlR,tetR
Construct size (kb): 6.900000095367432
Features: marker(s): ampR, cmlR, tetR
replicon: pMB1
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.9; HindIII--4.65, 3.05.
This is an unstable, tetracycline-resistant mutant of pHV14.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Primrose SB, Ehrlich SD. Isolation of plamid deletion mutants and study of their instability. Plasmid 6: 193-201, 1981. PubMed: 7029580

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet