pCB101 (ATCC® 39020)

Applications: shuttle vector  /  Depositors: Kyowa Ferm. Ind. Co., Ltd., S Wakaki, Kyowa Ferm. Ind. Co., Ltd.

Permits and Restrictions

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Designations pCB101
Depositors Kyowa Ferm. Ind. Co., Ltd., S Wakaki, Kyowa Ferm. Ind. Co., Ltd.
Biosafety Level 1
Host
Distribution host: Corynebacterium glutamicum LA103
Vector Information
Size (kb): 11.3000001907348600
Vector: pCB101 (plasmid)
Construction: pCG11 (ATCC 39022), pUB110 (ATCC 37015)
Marker(s):kanR,spcR
Construct size (kb): 11.30000019073486
Features: marker(s): kanR, spcR
replicon: pCG1, pUB110
Applications
shuttle vector
Comments
The original vials from the depositor are being used for distribution.
Cloning into the BglII site inactivates kanR.
This was constructed by ligating pCG11 (ATCC 39022) linearized with BglII to pUB110 linearized with BamHI.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C
References

Katsumata R, et al. Novel vector plasmids. US Patent 4,710,471 dated Dec 1 1987

Shipped frozen
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet