pMA200U (ATCC® 37833)

Applications: expression vectorvector containing primer sites useful for sequencingvector permitting production of single-stranded DNA  /  Depositors: NE Dixon

Permits and Restrictions

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Designations pMA200U
Depositors NE Dixon
Biosafety Level 1
Vector Information
Size (kb): 4.1519999504089360
Vector: pMA200U (phagemid)
Promoters: Promoter lambda PL
Construction: pCE30 (ATCC 37830), pTZ18U
Marker(s):ampR
Construct size (kb): 4.151999950408936
Features: marker(s): ampR
promoter: lambda PL, lambda PR
replicon: pMB1, f1
repressor gene: cI857
Applications
expression vector
vector containing primer sites useful for sequencing
vector permitting production of single-stranded DNA
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.3; BamHI/EcoRI--4.3; BglI--3.0, 1.3.
Upon infection with M13KO7 (ATCC 37468), an M13-sensitive host strain containing a pMA200U derivative produces packaged single-stranded plasmid DNA complementary to the M13 universal primer.
If the insert is oriented correctly for transcription from the lambda promoters, the single-stranded plasmid DNA will contain the coding strand.
Expression vector encoding cI857. Contains primer sites useful for sequencing and permits production of ssDNA.
Constructed by replacing the 1.118 kb BglI fragment of pCE30 with the analogous 1.27 kb fragment of pTZ18U.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Elvin CM, et al. Modified bacteriophage lambda promoter vectors for overproduction of proteins in Escherichia coli. Gene 87: 123-126, 1990. PubMed: 2139621

Shipped freeze-dried
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