pCYM1-11 (ATCC® 37828)

Applications: YE-type (episomal) shuttle vectorexpression vectorshuttle vector  /  Depositors: JH Camonis

Permits and Restrictions

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Designations pCYM1-11
Depositors JH Camonis
Biosafety Level 1
Vector Information
Size (kb): 5.6389999389648440
Vector: pCYM1-11 (plasmid)
Promoters: Promoter SV40 early
Construction: pCYM1 (ATCC 37822), M13mp11
Marker(s):URA3,ampR
Construct size (kb): 5.638999938964844
Features: marker(s): ampR, URA3
promoter: SV40 early
replicon: pMB1, 2 micron
terminator: SV40
Applications
YE-type (episomal) shuttle vector
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; BamHI/PvuII--5.2, 0.5; EcoRI--5.6.
Shuttle expression vector.
Permits expression in both S. cerevisiae and mouse cells.
The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter.
Constructed by replacing the HindIII/EcoRI fragment of pCYM1 with the HindIII/EcoRI multiple cloning site from M13mp11. The translation start codon has been removed by this process.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Camonis JH, et al. Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells. Gene 86: 263-268, 1990. PubMed: 2108907

Shipped freeze-dried
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