pCYM03 (ATCC® 37827)

Applications: YE-type (episomal) shuttle vectorexpression vectorreporter constructshuttle vector  /  Depositors: JH Camonis

Permits and Restrictions

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Designations pCYM03
Depositors JH Camonis
Biosafety Level 1
Vector Information
Size (kb): 9.5909996032714840
Vector: pCYM03 (plasmid)
Promoters: Promoter SV40 early
Construction: pCYM3 (ATCC 37826), pMC1403
Marker(s):TRP1,ampR,URA3d
Construct size (kb): 9.590999603271484
Features: insert detection: lacZ'
marker(s): ampR, TRP1, URA3d
promoter: SV40 early
replicon: pMB1, 2 micron
terminator: SV40
Applications
YE-type (episomal) shuttle vector
expression vector
reporter construct
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/ClaI--4.9, 3.5, 0.76, 0.48; EcoRI--9.4; SmaI--9.4.
URA3d is a modified URA3 gene which is not efficiently expressed and requires a large number of plasmids to complement a ura3 mutation.
Shuttle expression vector which includes a reporter group.
Expresses beta-galactosidase activity in E. coli, S. cerevisiae, and mouse cells, and thus can be used to calibrate transformation efficiency.
Expression can be regulated by using the medium to modulate copy number.
Also permits blue/white detection in E. coli.
The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter.
Constructed by inserting a 3.048 kb EcoRI/DraI fragment from pMC1403 into pCYM3 cut with BamHI (nt 427), linkered to add an EcoRI site, and cut with BalI (nt 702).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Camonis JH, et al. Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells. Gene 86: 263-268, 1990. PubMed: 2108907

Shipped freeze-dried
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