pCYM0 (ATCC® 37820)

Applications: YE-type (episomal) shuttle vectorexpression vectorshuttle vector  /  Depositors: JH Camonis

Permits and Restrictions

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Designations pCYM0
Depositors JH Camonis
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.3600001335144040
Vector: pCYM0 (plasmid)
Promoters: Promoter SV40 early
Construction: pSV2-cat, pUT322
Construct size (kb): 6.360000133514404
Features: marker(s): ampR, URA3
promoter: SV40 early
replicon: pMB1, 2 micron
terminator: SV40
YE-type (episomal) shuttle vector
expression vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BamHI/PvuII--5.6, 0.5; EcoRI--6.0.
Shuttle expression vector.
Permits expression in both S. cerevisiae and mouse cells.
The BamHI and EcoRI sites are 3' of the unique available in-phase initiation codon under control of the SV40 early promoter.
Constructed by inserting the PvuII/BamHI fragment from pSV2-cat (SV40 promoter, small-t intron, polyadenylation signal, inactive CAT) into pUT322 (2 micron origin, URA3).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Camonis JH, et al. Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells. Gene 86: 263-268, 1990. PubMed: 2108907

Shipped freeze-dried