pCYM0 (ATCC® 37820)

Applications: YE-type (episomal) shuttle vectorexpression vectorshuttle vector  /  Depositors: JH Camonis

Permits and Restrictions

View Permits

Designations pCYM0
Depositors JH Camonis
Biosafety Level 1
Vector Information
Size (kb): 6.3600001335144040
Vector: pCYM0 (plasmid)
Promoters: Promoter SV40 early
Construction: pSV2-cat, pUT322
Marker(s):URA3,ampR
Construct size (kb): 6.360000133514404
Features: marker(s): ampR, URA3
promoter: SV40 early
replicon: pMB1, 2 micron
terminator: SV40
Applications
YE-type (episomal) shuttle vector
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BamHI/PvuII--5.6, 0.5; EcoRI--6.0.
Shuttle expression vector.
Permits expression in both S. cerevisiae and mouse cells.
The BamHI and EcoRI sites are 3' of the unique available in-phase initiation codon under control of the SV40 early promoter.
Constructed by inserting the PvuII/BamHI fragment from pSV2-cat (SV40 promoter, small-t intron, polyadenylation signal, inactive CAT) into pUT322 (2 micron origin, URA3).
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Camonis JH, et al. Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells. Gene 86: 263-268, 1990. PubMed: 2108907

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet