pHPS9R (ATCC® 37818)

Applications: Plasmid was not verified by restriction analysis.Recommended for pHPS9 (ATCC 37816, 37817) to permit alpha-complementation and plasmid marker rescue transformation for transformation of competent cells with plasmid monomers.pHPS9R serves as the helper plasmid.  /  Depositors: S Bron

Permits and Restrictions

View Permits

Designations pHPS9R
Depositors S Bron
Biosafety Level 1
Vector Information
Size (kb): 4.0999999046325680
Vector: pHPS9R (plasmid)
Construction: pHP13-2
Marker(s):eryR
Construct size (kb): 4.099999904632568
Features: marker(s): eryR
replicon: pMB1, pTA1060
Applications
Plasmid was not verified by restriction analysis.
Recommended for pHPS9 (ATCC 37816, 37817) to permit alpha-complementation and plasmid marker rescue transformation for transformation of competent cells with plasmid monomers.
pHPS9R serves as the helper plasmid.
Comments
Plasmid was not verified by restriction analysis. Strain was checked for the following phenotypes: showed growth on LB + erythromycin (150 mcg/ml), white colonies on Xgal + IPTG, white colonies on Xgal + IPTG + erythromycin (150 mcg/ml).
Recommended for pHPS9 (ATCC 37816, 37817) to permit alpha-complementation and plasmid marker rescue transformation for transformation of competent cells with plasmid monomers. pHPS9R serves as the helper plasmid.
Differs from pHPS9 (ATCC 37816, 37817) by partial removal of chloramphenicol resistance sequence (downstream of the NcoI site) and removal of the P59 promoter and sequences encoding the cat86::lacZalpha fusion.
Media Medium 2305: 1065 plus erythromycin (150 mcg/ml)
Growth Conditions
Temperature: 37.0°C
References

Haima P, et al. Novel plasmid marker rescue transformation system for molecular cloning in Bacillus subtilis enabling direct selection of recombinants. Mol. Gen. Genet. 223: 185-191, 1990. PubMed: 2123518

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet