pMMB206 in Escherichia coli (ATCC® 37808)

Applications: expression vectorshuttle vector  /  Depositors: V Morales, National Research Council, Plant Biotechnology Institute, Saskatoon, Sask., Canada

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Designations pMMB206 in Escherichia coli
Depositors V Morales, National Research Council, Plant Biotechnology Institute, Saskatoon, Sask., Canada
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host Escherichia coli TB1
Vector Information
Size (kb): 9.31
Vector: pMMB206 (plasmid)
Promoters: Promoter tac
Construction: pMMB190, pUC8
Marker(s): cmlR
Construct size (kb): 9.31
Features: insert detection: lacZ'
marker(s): cmlR
promoter: tac, lacUV5
replicon: oriV, oriT (IncQ)
repressor gene: lacIq
transcription terminator: rrnB
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.3; EcoRV--5.8, 3.6; SspI--3.9, 1.8, 1.6, 1.2, 0.9; EcoRI/MluI--8.4, 0.9.
Contains sequences encoding lacIq, a tandem tac lacUV5 promoter region, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Shows a low level of expression without induction.
Sequencing demonstrated that there are 3 stop codons in frame with the truncated bla gene, terminating translation but permitting continued transcription and expression of the cat gene.
Media ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37°C
References

Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347

Shipped frozen