pMMB190 (ATCC® 37807)

Applications: expression vectorshuttle vector  /  Depositors: VM Morales

Permits and Restrictions

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Designations pMMB190
Depositors VM Morales
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 9.0640001296997070
Vector: pMMB190 (plasmid)
Promoters: Promoter tac
Construction: pMMB66EH, pUC8
Marker(s):ampR
Construct size (kb): 9.064000129699707
Features: insert detection: lacZ'
marker(s): ampR
promoter: tac, lacUV5
replicon: oriV, oriT (IncQ)
repressor gene: lacIq
transcription terminator: rrnB
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.1; EcoRV--5.3, 3.8; SspI--4.9, 1.8, 1.6, 1.0; EcoRI/MluI--8.2, 0.9.
Contains sequences encoding lacIq, a tandem tac lacUV5 promoter region, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Shows a low level of expression without induction.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli  containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37807