pMMB190 (ATCC® 37807)

Applications: expression vectorshuttle vector  /  Depositors: VM Morales

Permits and Restrictions

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Designations pMMB190
Depositors VM Morales
Biosafety Level 1
Vector Information
Size (kb): 9.0640001296997070
Vector: pMMB190 (plasmid)
Promoters: Promoter tac
Construction: pMMB66EH, pUC8
Marker(s):ampR
Construct size (kb): 9.064000129699707
Features: insert detection: lacZ'
marker(s): ampR
promoter: tac, lacUV5
replicon: oriV, oriT (IncQ)
repressor gene: lacIq
transcription terminator: rrnB
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.1; EcoRV--5.3, 3.8; SspI--4.9, 1.8, 1.6, 1.0; EcoRI/MluI--8.2, 0.9.
Contains sequences encoding lacIq, a tandem tac lacUV5 promoter region, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Shows a low level of expression without induction.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli  containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37807