pDdGal-16 (ATCC® 37771)

Applications: promoter-cloning vectorshuttle vector  /  Depositors: AJ Harwood

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Designations pDdGal-16
Depositors AJ Harwood
Biosafety Level 1
Vector Information
Size (kb): 8.5000000000000000
Vector: pDdGal-16 (plasmid)
Promoters: Promoter none
Construction: pDdlac-1, pB10TP2
Marker(s):ampR
Construct size (kb): 8.5
Features: insert detection: lacZ
marker(s): ampR
promoter: none
replicon: pMB1
terminator: actin 8
Applications
promoter-cloning vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--8.5; BamHI--8.5; BglII--8.5.
A promoter-cloning plasmid vector using expression of beta-galactosidase as the reporter in Dictyostelium.
pDdGal-15 (ATCC 37770), pDdGal-16 (ATCC 37771) and pDdGal-17 (ATCC 37772) differ in their polylinker sequences. Each contains a BglII site in a different reading frame relative to the lacZ gene.
The lacZ gene and Dictyostelium actin 8 terminator from pDdlac-1 were inserted into a derivative of pB10TP2 from which the f1 origin and and a lacZ fragment had been removed.
The neo gene is regulated by an actin 6 promoter and a CP1 terminator.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Harwood AJ, Drury L. New vectors for expression of the E. coli lacZ gene in Dictyostelium. Nucleic Acids Res. 18: 4292, 1990. PubMed: 2198542

Shipped freeze-dried
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