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Size (kb): 8.5000000000000000
Vector: pDdGal-16 (plasmid)
Promoters: Promoter none
Construction: pDdlac-1, pB10TP2
Construct size (kb): 8.5
Features: insert detection: lacZ
terminator: actin 8
Restriction digests of the clone give the following sizes (kb): EcoRI--8.5; BamHI--8.5; BglII--8.5.
A promoter-cloning plasmid vector using expression of beta-galactosidase as the reporter in Dictyostelium.
pDdGal-15 (ATCC 37770)
, pDdGal-16 (ATCC 37771)
and pDdGal-17 (ATCC 37772)
differ in their polylinker sequences. Each contains a BglII site in a different reading frame relative to the lacZ gene.
The lacZ gene and Dictyostelium actin 8 terminator from pDdlac-1 were inserted into a derivative of pB10TP2 from which the f1 origin and and a lacZ fragment had been removed.
The neo gene is regulated by an actin 6 promoter and a CP1 terminator.
Harwood AJ, Drury L. New vectors for expression of the E. coli lacZ gene in Dictyostelium. Nucleic Acids Res. 18: 4292, 1990. PubMed: 2198542