Size (kb): 7.0999999046325680
Vector: YIp355 (plasmid)
Promoters: Promoter none
Construction: YEp355, YIp352
Construct size (kb): 7.099999904632568
Features: insert detection: lacZ
marker(s): ampR, URA3
YI-type (integrating) shuttle vector
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-3; SmaI-1; BamHI-1; SalI-1; PstI-2; HindIII-3.
Restriction digests of the clone give the following sizes (kb): EcoRI--7.1; BamHI--7.1; SalI--7.1; PstI--7.1; HindIII--7.1.
One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37743
- 37745) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5' G AAT TCC CGG GGA TCC GTC GAC CTG CAG CCA AGC TTC GAT CCC 3'. The sequence begins with the first nucleotide of the MCS and ends with the CCC for amino acid 8 of beta-galactosidase.
Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915