YEp358R (ATCC® 37739)

Applications: YE-type (episomal) shuttle vectorpromoter-cloning vectorshuttle vector  /  Depositors: CJ Lusty

Permits and Restrictions

View Permits

Designations YEp358R
Depositors CJ Lusty
Biosafety Level 1
Vector Information
Size (kb): 8.0000000000000000
Vector: YEp358R (plasmid)
Promoters: Promoter none
Construction: YEp358 (ATCC 37733), pUC18
Marker(s):URA3,ampR
Construct size (kb): 8.0
Features: insert detection: lacZ
marker(s): ampR, URA3
promoter: none
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
promoter-cloning vector
shuttle vector
Comments
Cloning into the HindIII, SphI, PstI, SalI, or XbaI sites leads to a TAG stop codon within the downstream XbaI site of the multiple cloning region.
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-2; SphI-3; PstI-3; SalI-2; XbaI-2; BamHI-2; SmaI-3; KpnI-3; SacI-3; EcoRI-2. The SacI site is not unique.
Restriction digests of the clone give the following sizes (kb): HindIII--8.0; EcoRI--8.0; PstI--8.0.
One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37737 - 37739) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'AA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CCC GGG TAC CGA GCT CGA ATT CCC AGC TTC GAT CCC3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet