Size (kb): 8.0000000000000000
Vector: YEp357 (plasmid)
Promoters: Promoter none
Construction: YEp354 (ATCC 37726)
Construct size (kb): 8.0
Features: insert detection: lacZ
marker(s): ampR, URA3
replicon: pMB1, 2 micron
YE-type (episomal) shuttle vector
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-1; SacI-2; KpnI-2; SmaI-1; BamHI-1; XbaI-1; SalI-1; PstI-2; SphI-2; HindIII-1. The SacI site is not unique.
Restriction digests of the clone give the following sizes (kb): EcoRI--8.0; BamHI--8.0; SalI--8.0; PstI--8.0.
One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37731
- 37733) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'GAA TTC GAG CTC GGT ACC CGG GGA TCC TCT AGA GTC GAC CTG CAG GCA TGC AAG CTT GCT CCC 3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915