YEp356 (ATCC® 37731)

Applications: YE-type (episomal) shuttle vectorpromoter-cloning vectorshuttle vector  /  Depositors: CJ Lusty

Permits and Restrictions

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Designations YEp356
Depositors CJ Lusty
Biosafety Level 1
Vector Information
Size (kb): 8.0000000000000000
Vector: YEp356 (plasmid)
Promoters: Promoter none
Construction: YEp353 (ATCC 37725), pUC18
Marker(s):URA3,ampR
Construct size (kb): 8.0
Features: insert detection: lacZ
marker(s): ampR, URA3
promoter: none
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
promoter-cloning vector
shuttle vector
Comments
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-2; SacI-3; KpnI-3; SmaI-2; BamHI-2; XbaI-2; SalI-2; PstI-3; SphI-3; HindIII-2. The SacI site is not unique.
Cloning into the EcoRI, SacI, KpnI SmaI, BamHI, or XbaI sites leads to a TAG stop codon within the downstream XbaI site of the multiple cloning region.
Restriction digests of the clone give the following sizes (kb): PstI--8.0; HindIII--8.0; SalI--8.0; EcoRI--8.0; BamHI--8.0.
One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37731 - 37733) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'GA ATT CGA GCT CGG TAC CCG GGG ATC CTC TAG AGT CGA CCT GCA GGC ATG CAA GCT TGC GAT CCC 3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915

Shipped freeze-dried
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