pJ6omega (ATCC® 37723)

Applications: expression vectorshuttle vector  /  Depositors: H Land

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Designations pJ6omega
Depositors H Land
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.5000000000000000
Vector: pJ6omega (plasmid)
Promoters: Promoter rat beta-actin
Construction: pBR322, SV40, rat beta-actin
Marker(s):ampR
Construct size (kb): 3.5
Features: marker(s): ampR
promoter: rat beta-actin
replicon: pMB1
terminator: SV40
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.5; HindIII--3.5; BamHI--3.5; SmaI--3.5; PvuI--2.15, 1.35.
The actin promoter has been shown to be expressed efficiently in undifferentiated cells in which transcription from other promoters (SV40 early, Mo MuLV LTR, MMTV LTR) is severely repressed.
A mammalian expression vector for expression of a sequence cloned into the polylinker site under the control of the rat beta actin promoter.
Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), and the rat beta-actin promoter (-340 to +10).
Media ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Growth Conditions
Temperature: 37.0°C
References

Morgenstern JP, Land H. A series of mammalian expression vectors and characterisation of their expression of a reporter gene in stably and transiently transfected cells. Nucleic Acids Res. 18: 1068, 1990. PubMed: 2156225

Tsutsumi M, et al. Cloning and functional expression of a mouse gonadotropin-releasing hormone receptor. Mol. Endocrinol. 6: 1163-1169, 1992. PubMed: 1324422

Shipped frozen
Shipping Information Distributed: freeze-dried
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