pJ4omega (ATCC® 37720)

Applications: expression vectorshuttle vector  /  Depositors: H Land

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Designations pJ4omega
Depositors H Land
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.7000000476837160
Vector: pJ4omega (plasmid)
Promoters: Promoter Mo-MuLV LTR
Construction: pBR322, SV40 Mo-MuLV
Marker(s):ampR
Construct size (kb): 3.700000047683716
Features: marker(s): ampR
promoter: Mo-MuLV LTR
replicon: pMB1
terminator: SV40
enhancer: Mo-MuLV LTR
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7; HindIII--3.7; BamHI--3.7; SmaI--3.7; PvuI--2.2, 1.5.
A mammalian expression vector for expression of a sequence cloned into the polylinker site under the control of the Mo-MuLV LTR promoter.
Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), and Mo-MuLV LTR.
Media ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Growth Conditions
Temperature: 37.0°C
References

Morgenstern JP, Land H. A series of mammalian expression vectors and characterisation of their expression of a reporter gene in stably and transiently transfected cells. Nucleic Acids Res. 18: 1068, 1990. PubMed: 2156225

Hartmut Land, personal communication

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37720