pTacNeo1.2 [CMCC 2296] (ATCC® 37687)

Applications: expression vectorshuttle vectorvector permitting construction of fusion proteins  /  Depositors: JC Hunter-Cevera

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Designations pTacNeo1.2 [CMCC 2296]
Depositors JC Hunter-Cevera
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.4400000572204590
Vector: pTacNeo1.2 (plasmid)
Promoters: Promoter tac
Construction: pBR322, TAC promoter, lacZ, modified APH(3')-I
Construct size (kb): 5.440000057220459
Features: marker(s): ampR
promoter: tac
replicon: pMB1
expression vector
shuttle vector
vector permitting construction of fusion proteins
Restriction digests of the clone give the following sizes (kb): BamHI--5.45; XhoI--5.45; EcoRI--5.45; PvuI--4.0, 1.45; PstI--3.1, 2.35.
This is an expression vector capable of producing a beta-galactosidase: insert: APH(3')-I fusion protein.
The start of the modified APH(3')-I is followed by the eleventh codon (serine) of APH(3')-I. Codons 2-10 have been deleted.
The vector contains pBR322 (EcoRI-SphI), a TAC promoter, the first 8 amino-terminal codons of beta-galactosidase, and a gene encoding a modified aminoglycoside phosphotransferase (3')-I (APH(3')-I).
The upstream translation of LacZ is out of frame with APH(3')-I.
Sequences from the TAA stop codon of APH(3')-I to the immediate downstream PvuII site encode a portion of Tn601. Sequences from the PvuII site to the PstI site are derived from Tn5.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Jennie C Hunter-Cevera, personal communication

Shipped freeze-dried