YEp352 (ATCC® 37673)

Applications: YE-type (episomal) shuttle vectorshuttle vector  /  Depositors: A Tzagoloff

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Designations YEp352
Depositors A Tzagoloff
Biosafety Level 1
Vector Information
Size (kb): 5.1799998283386230
Vector: YEp352 (plasmid)
Promoters: Promoter lac
Construction: pUC18, 2 micron, URA3
Marker(s):URA3,ampR
Construct size (kb): 5.179999828338623
Features: insert detection: lacZ'
marker(s): ampR, URA3
promoter: lac
replicon: pMB1, 2 micron
terminator: none
enhancer: none
Applications
YE-type (episomal) shuttle vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.3; XbaI--5.3; PstI--5.3; HindIII--5.3.
This is a Escherichia coli/yeast episomal shuttle vector with multiple restriction sites for cloning.
Constructed by filling in and blunt-end ligating pUC18 linearized at NdeI, a 1.4 kb RsaI fragment of the 2 micron circle, and a 1.1 kb HindIII/SmaI fragment containing URA3. A XbaI site in 2u and a PstI site in URA3 were removed.
The yeast genomic sequence is in nt 286-1383 (where the A of the ATG of lacZ' is number 1), the 2 micron sequence goes from nt 1384-2768, and pUC18 includes nt 2769-285.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Hill JE, et al. Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2: 163-167, 1986. PubMed: 3333305

Alexander Tzagoloff, personal communication

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Shipping Information Distributed: freeze-dried
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