YEp352 (ATCC® 37673)

Applications: YE-type (episomal) shuttle vectorshuttle vector  /  Depositors: A Tzagoloff

Permits and Restrictions

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Designations YEp352
Depositors A Tzagoloff
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.1799998283386230
Vector: YEp352 (plasmid)
Promoters: Promoter lac
Construction: pUC18, 2 micron, URA3
Marker(s):URA3,ampR
Construct size (kb): 5.179999828338623
Features: insert detection: lacZ'
marker(s): ampR, URA3
promoter: lac
replicon: pMB1, 2 micron
terminator: none
enhancer: none
Applications
YE-type (episomal) shuttle vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.3; XbaI--5.3; PstI--5.3; HindIII--5.3.
This is a Escherichia coli/yeast episomal shuttle vector with multiple restriction sites for cloning.
Constructed by filling in and blunt-end ligating pUC18 linearized at NdeI, a 1.4 kb RsaI fragment of the 2 micron circle, and a 1.1 kb HindIII/SmaI fragment containing URA3. A XbaI site in 2u and a PstI site in URA3 were removed.
The yeast genomic sequence is in nt 286-1383 (where the A of the ATG of lacZ' is number 1), the 2 micron sequence goes from nt 1384-2768, and pUC18 includes nt 2769-285.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Hill JE, et al. Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2: 163-167, 1986. PubMed: 3333305

Alexander Tzagoloff, personal communication

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