pHP45omega-Tc [JF297] (ATCC® 37656)

Applications: integrating vectorshuttle vectorvector permitting positive selection for integrationvector useful for interposon mutagenesis  /  Depositors: J Frey

Permits and Restrictions

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Designations pHP45omega-Tc [JF297]
Depositors J Frey
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.4800000190734860
Vector: pHP45omega-Tc (plasmid)
Construction: pHP45:omega, pBR322 tetR
Construct size (kb): 4.480000019073486
Features: marker(s): ampR, tetR
replicon: pMB1
terminator: T4D gene 32
integrating vector
shuttle vector
vector permitting positive selection for integration
vector useful for interposon mutagenesis
Restriction digests of the clone give the following sizes (kb): EcoRI--2.5, 2.4; HindIII--2.45, 2.3; SalI--4.9.
Because resistance levels may vary, it is important to determine the proper antibiotic concentration for each strain or isolate.
One of a series (ATCC 37653 through 37657) of vectors for in vitro mutagenesis (via the omega interposon) of gram-negative genera.
pHP45:omega was cut with BssHII + SphI (removing the spcR/strR gene) and the ends were made blunt. This was ligated to a blunt-ended 1.4 kb HindIII/AvaI fragment of pBR322 containing the tetracycline resistance gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Fellay R, et al. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52: 147-154, 1987. PubMed: 3038679

Frey J, Krisch HM. Omega mutagenesis in Gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species. Gene 36: 143-150, 1985. PubMed: 2998930

Shipped freeze-dried
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