pUMSV0CAT (ATCC® 37651)

Applications: promoter-cloning vectorshuttle vector  /  Depositors: K Kurachi

Permits and Restrictions

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Designations pUMSV0CAT
Depositors K Kurachi
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.5000000000000000
Vector: pUMSV0CAT (plasmid)
Promoters: Promoter none
Construction: pSV0cat, UMS of c-mos
Marker(s):ampR,cmlS
Construct size (kb): 5.5
Features: insert detection: CAT
marker(s): ampR, cmlS
promoter: none
replicon: pMB1
Applications
promoter-cloning vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 2.0; SmaI--5.7.
The UMS sequences reduce background CAT transcription from the vector to levels approaching mock-transfected cells.
A shuttle vector for assessing weak transcriptional activity of a cloned promoter using a CAT reporter gene.
A 1023 bp SacI/XbaI fragment containing the poly(A) signal of mouse c-mos (UMS) was cloned into the NdeI site of pSV0cat. The NdeI site is 56 bp upstream of the SmaI cloning site. Constructed by J-P. Salier and K. Kurachi.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Salier JP, Kurachi K. A CAT expression vector with virtually no background: pUMSV0CAT. BioTechniques 7: 30-31, 1989. PubMed: 2629830

Shipped freeze-dried