pUMSV0CAT (ATCC® 37651)

Applications: promoter-cloning vectorshuttle vector  /  Depositors: K Kurachi

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Designations pUMSV0CAT
Depositors K Kurachi
Biosafety Level 1
Vector Information
Size (kb): 5.5000000000000000
Vector: pUMSV0CAT (plasmid)
Promoters: Promoter none
Construction: pSV0cat, UMS of c-mos
Marker(s):ampR,cmlS
Construct size (kb): 5.5
Features: insert detection: CAT
marker(s): ampR, cmlS
promoter: none
replicon: pMB1
Applications
promoter-cloning vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 2.0; SmaI--5.7.
The UMS sequences reduce background CAT transcription from the vector to levels approaching mock-transfected cells.
A shuttle vector for assessing weak transcriptional activity of a cloned promoter using a CAT reporter gene.
A 1023 bp SacI/XbaI fragment containing the poly(A) signal of mouse c-mos (UMS) was cloned into the NdeI site of pSV0cat. The NdeI site is 56 bp upstream of the SmaI cloning site. Constructed by J-P. Salier and K. Kurachi.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Salier JP, Kurachi K. A CAT expression vector with virtually no background: pUMSV0CAT. BioTechniques 7: 30-31, 1989. PubMed: 2629830

Shipped freeze-dried
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