pGCAT-C (ATCC® 37641)

Applications: expression vectorpromoter-cloning vectorshuttle vectorvector permitting RNA synthesis in vitro  /  Depositors: T Frebourg, O Brison

Designations pGCAT-C
Depositors T Frebourg, O Brison
Biosafety Level 1
Vector Information
Size (kb): 4.5500001907348630
Vector: pGCAT-C (plasmid)
Promoters: Promoter T7
Construction: pSB1, pGEM4
Marker(s):ampR
Construct size (kb): 4.550000190734863
Features: insert detection: CAT
marker(s): ampR
promoter: SP6, T7
replicon: pMB1
Applications
expression vector
promoter-cloning vector
shuttle vector
vector permitting RNA synthesis in vitro
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--3.0, 1.5; HincII--3.0, 1.5; EcoRI--4.25, 0.3.
A plasmid shuttle vector for investigating promoter and/or enhancer elements. Has a polylinker upstream of reporter CAT gene, SP6 and T7 promoters for in vitro RNA synthesis and low basal CAT expression in eukaryotic cells.
The sequence at the junction of the multiple cloning site and CAT is GAATTCGAGC and at the junction of SV40 and SP6 is GGATCAGCTT.
The polylinker is in opposite orientation in pGCAT-A (ATCC 37640) and pGCAT-C (ATCC 37641).
BamHI/HindIII fragment of pSB1 (CAT) was cloned into the HindIII site of pGEM-4. 2 hrs after ligation began the remaining BamHI and HindIII sites were filled in. Contains SV40 polyadenylation site and donor and acceptor sites of SV40 small-t intron.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Frebourg T, Brison O. Plasmid vectors with multiple cloning sites and cat-reporter gene for promoter cloning and analysis in animal cells. Gene 65: 315-318, 1988. PubMed: 2842234

Thierry Frebourg, personal communication

Shipped freeze-dried
Product Sheet
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Product Sheet