YIp5-32phocat (ATCC® 37629)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: H Feldmann

Permits and Restrictions

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Designations YIp5-32phocat
Depositors H Feldmann
Biosafety Level 1
Vector Information
Size (kb): 6.9099998474121090
Vector: YIp5-32phocat (plasmid)
Promoters: Promoter PHO5
Construction: YIp5, YRp7-32phocat (ATCC 37625)
Marker(s):URA3,ampR
Construct size (kb): 6.909999847412109
Features: marker(s): ampR, URA3
promoter: PHO5
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.6, 0.64; PstI--3.85, 3.3; PvuII--3.7, 3.4.
The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 4920 bp SalI/HindIII fragment of YIp5 was ligated to a 1985 bp HindIII/SalI fragment of YRp7-32phocat (ATCC 37625).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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