YRp7-32phocat (ATCC® 37625)

Applications: YR-type (replicating) shuttle vectorshuttle vector  /  Depositors: H Feldmann

Permits and Restrictions

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Designations YRp7-32phocat
Depositors H Feldmann
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.5300002098083500
Vector: YRp7-32phocat (plasmid)
Promoters: Promoter PHO5
Construction: YRp7-32cat (ATCC 37624), PHO5
Construct size (kb): 6.53000020980835
Features: marker(s): ampR, TRP1
promoter: PHO5
replicon: pMB1, ARS1
YR-type (replicating) shuttle vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 1.37, 0.55; PstI--5.5, 1.3; PvuII--4.7, 2.15.
The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 570 bp SalI/EcoRI fragment containing the PHO5 promoter was ligated to a 5965 bp BamHI/SalI fragment of YRp7-32cat (ATCC 37624).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257

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