pYAC-RC (ATCC® 37610)

Applications: YL-type (YAC) shuttle vectorshuttle vectorvector for constructing genomic libraries  /  Depositors: D Marchuk

Permits and Restrictions

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Designations pYAC-RC
Depositors D Marchuk
Biosafety Level 1
Vector Information
Size (kb): 11.6999998092651400
Vector: pYAC-RC (YAC)
Promoters: Promoter none
Construction: pYAC3, polylinker
Marker(s):TRP1,URA3,ampR,HIS3,SUP4
Construct size (kb): 11.69999980926514
Features: marker(s): ampR, HIS3, SUP4, TRP1, URA3
promoter: none
replicon: pMB1, ARS1
centromere: CEN4
Applications
YL-type (YAC) shuttle vector
shuttle vector
vector for constructing genomic libraries
Comments
Digestion with HindIII gives the following sizes (kb): 3.5, 3.0, 1.9, 1.4(doublet), small fragment. If any telomere DNA has been deleted, the doublet will be resolved and a new band will appear.
Restriction digests of the clone give the following sizes (kb): HindIII--3.7, 3.0, 1.9, 1.47; SalI--12.0.
An occasional isolate will spontaneously delete small amounts of telomere sequence. Check by digesting the plasmid with HindIII.
Due to ligation compatibility with sites in the polylinker, DNA fragments from BssHII, EagI, NaeI, NarI, NruI, SmaI and XhoI digests may also be cloned in this vector.
A yeast artificial chromosome vector for genomic library construction using DNA digested with infrequent restriction enzymes.
Constructed by removing the ClaI and SalI sites from pYAC3 and inserting a polylinker in the SnaBI site within SUP4. SUP4 still functions.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Marchuk D, Collins FS. pYAC-RC, a yeast artificial chromosome vector for cloning DNA cut with infrequently cutting restriction endonucleases. Nucleic Acids Res. 16: 7743, 1988. PubMed: 3045765

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Shipping Information Distributed: freeze-dried
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