EBO-pSV2-neo (ATCC® 37593)

Applications: expression vectorshuttle vector  /  Depositors: RF Margolskee

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Designations EBO-pSV2-neo
Depositors RF Margolskee
Biosafety Level 1
Vector Information
Size (kb): 13.3599996566772500
Vector: EBO-pSV2-neo (plasmid)
Promoters: Promoter SV40 late
Construction: pSV2neo, p220-1 (EBV oriP, EBNA-1, hph)
Marker(s):G418R,ampR,hygR
Construct size (kb): 13.35999965667725
Features: marker(s): ampR, hygR, G418R
promoter: SV40 late
replicon: pMB1, EBV oriP
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--13.4; SfiI--7.5, 6.1; PstI--7.4, 2.3, 1.5, 1.0; SacI--13.4; BamHI--6.8, 5.4; HindIII--13.4; BglII--6.6.
pcD cDNA libraries can be converted into EBO-pcD libraries by ligating the 7.4 kb SfiI fragment of EBO-pSV2-neo (containing oriP, EBNA-1, hph) into SfiI-linearized, phosphatased, pcD DNA.
A selectable, stably replicating shuttle vector for expression of cDNAs in eukaryotic cells. Maintained episomally.
The vector contains EBV oriP and EBNA to ensure replication as episomes in human or simian cells, even if the cell line is not EBNA positive.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Margolskee RF, et al. Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells. Mol. Cell. Biol. 8: 2837-2847, 1988. PubMed: 2841588

Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549

Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831

Okayama H, Berg P. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell. Biol. 3: 280-289, 1983. PubMed: 6300662

Mulligan RC, et al. Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature 277: 108-114, 1979. PubMed: 215915

Shipped freeze-dried
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