pHHluc Plasmid in Escherichia coli (ATCC® 37581)

Applications:  expression vector  shuttle vector  /  Depositors: SK Nordeen

Permits and Restrictions

View Permits View Restrictions

Designations pHHluc Plasmid in Escherichia coli
Depositors SK Nordeen
Biosafety Level 1
Vector Information
Size (kb): 6.19
Vector: pHHluc (plasmid)
Promoters: Promoter MMTV
Construction: pXP2, MMTV promoter
Marker(s):ampR
Construct size (kb): 6.19
Features: insert detection: luciferase
marker(s): ampR
promoter: MMTV
replicon: pMB1
terminator: none
enhancer: HRE
Applications
 expression vector
 shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; HindIII--6.6; SalI--6.6; matches depositor's DNA.
This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California.
There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts.
One of a series of luciferase vectors (ATCC 37574-37584) for assaying promoter activity in mammalian cells.
The insert contains bp -223 to +105 of the MMTV promoter with a functional HRE.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Nordeen SK. Luciferase reporter gene vectors for analysis of promoters and enhancers. BioTechniques 6: 454-458, 1988. PubMed: 2908509

de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727

Steven K Nordeen, personal communication

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Restrictions This product's use is governed by a Label License. For information on purchasing a license to use this product for purposes other than those permitted in the Label License, please contact Promega.
Product Sheet
Product Sheet
Product Sheet