pSluc2* Plasmid in Escherichia coli (ATCC® 37574)

Applications:  promoter-cloning vector  shuttle vector  /  Depositors: SK Nordeen

Permits and Restrictions

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Designations pSluc2* Plasmid in Escherichia coli
Depositors SK Nordeen
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.1999998092651370
Vector: pSluc2* (plasmid)
Promoters: Promoter SV40
Construction: pSV232AL-Adelta5' derivative
Marker(s):ampR
Construct size (kb): 6.199999809265137
Features: insert detection: luciferase
marker(s): ampR
promoter: SV40
replicon: pMB1
terminator: none
enhancer: none
Applications
 promoter-cloning vector
 shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; HindIII--6.6; SalI--6.6; matches depositor's DNA.
There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts.
This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California.
One of a series of luciferase vectors (ATCC 37574-37584) for assaying promoter activity in mammalian cells.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Nordeen SK. Luciferase reporter gene vectors for analysis of promoters and enhancers. BioTechniques 6: 454-458, 1988. PubMed: 2908509

de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727

Steven K Nordeen, personal communication

Shipped freeze-dried
Restrictions This product's use is governed by a Label License. For information on purchasing a license to use this product for purposes other than those permitted in the Label License, please contact Promega.
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