pEMBL18-Not (Sma-) (ATCC® 37573)

Applications: vector for constructing genomic librariesvector permitting construction of fusion proteinsvector permitting production of single-stranded DNA  /  Depositors: A Craig

Permits and Restrictions

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Designations pEMBL18-Not (Sma-)
Depositors A Craig
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.9000000953674320
Vector: pEMBL18-Not (Sma-) (phagemid)
Promoters: Promoter lac
Construction: pEMBL18
Marker(s):ampR
Construct size (kb): 3.900000095367432
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1, f1
Applications
vector for constructing genomic libraries
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.9; NotI--4.0; EcoRI--3.9.
General purpose vector modified to include a NotI site to facilitate library construction and chromosome walking.
SmaI site in polylinker of pEMBL18 changed to a NotI site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Ehrich E, et al. A family of cosmid vectors with the multi-copy R6K replication origin. Gene 57: 229-237, 1987. PubMed: 2961651

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet