pEMBL18-Not (Sma-) (ATCC® 37573)

Applications: vector for constructing genomic librariesvector permitting construction of fusion proteinsvector permitting production of single-stranded DNA  /  Depositors: A Craig

Permits and Restrictions

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Designations pEMBL18-Not (Sma-)
Depositors A Craig
Biosafety Level 1
Vector Information
Size (kb): 3.9000000953674320
Vector: pEMBL18-Not (Sma-) (phagemid)
Promoters: Promoter lac
Construction: pEMBL18
Marker(s):ampR
Construct size (kb): 3.900000095367432
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1, f1
Applications
vector for constructing genomic libraries
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.9; NotI--4.0; EcoRI--3.9.
General purpose vector modified to include a NotI site to facilitate library construction and chromosome walking.
SmaI site in polylinker of pEMBL18 changed to a NotI site.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Ehrich E, et al. A family of cosmid vectors with the multi-copy R6K replication origin. Gene 57: 229-237, 1987. PubMed: 2961651

Shipped freeze-dried
Product Sheet
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