lambdaZd31 (ATCC® 37563)

Applications: expression vectorlambda vector allowing conversion to plasmidshuttle vectorvector for constructing cDNA libraries  /  Depositors: AJ Murphy

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Designations lambdaZd31
Depositors AJ Murphy
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 43.70
Vector: lambdaZd31 (phage, lambda - replacement)
Promoters: Promoter Mo-MLV LTR
Construction: Charon 30, pZIP-neoSV(x)1
Construct size (kb): 43.70
Features: marker(s): ampR, kanR, neoR, G418R
promoter: Mo-MLV LTR
replicon: lambda
terminator: 3' LTR
expression vector
lambda vector allowing conversion to plasmid
shuttle vector
vector for constructing cDNA libraries
Restriction digests of the clone give the following sizes (kb): EcoRI--19, 25.
The plasmid insert can be released by homologous recombination between the 2 LTRs after growth at low temperature in Escherichia coli RRI(pRK248), ATCC 47036.
A cell line suitable for transfection by released plasmid is Wgd5, ATCC CRL 1817.
Allows expression of cloned cDNA in mammalian cells; can be used for library transduction.
Contains Mo-MLV LTR for transcription of cDNA and neo; ori of SV40 and pBR322.

Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791

Shipped freeze-dried