lambdaZd31 (ATCC® 37563)

Applications: expression vectorlambda vector allowing conversion to plasmidshuttle vectorvector for constructing cDNA libraries  /  Depositors: AJ Murphy

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Designations lambdaZd31
Depositors AJ Murphy
Biosafety Level 1
Vector Information
Size (kb): 43.70
Vector: lambdaZd31 (phage, lambda - replacement)
Promoters: Promoter Mo-MLV LTR
Construction: Charon 30, pZIP-neoSV(x)1
Marker(s):G418R,ampR,kanR,neoR
Construct size (kb): 43.70
Features: marker(s): ampR, kanR, neoR, G418R
promoter: Mo-MLV LTR
replicon: lambda
terminator: 3' LTR
Applications
expression vector
lambda vector allowing conversion to plasmid
shuttle vector
vector for constructing cDNA libraries
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--19, 25.
The plasmid insert can be released by homologous recombination between the 2 LTRs after growth at low temperature in Escherichia coli RRI(pRK248), ATCC 47036.
A cell line suitable for transfection by released plasmid is Wgd5, ATCC CRL 1817.
Allows expression of cloned cDNA in mammalian cells; can be used for library transduction.
Contains Mo-MLV LTR for transcription of cDNA and neo; ori of SV40 and pBR322.
References

Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791

Shipped freeze-dried
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