pCHR81 (ATCC® 37535)

Applications: vector useful for transposon mutagenesis  /  Depositors: C Sasakawa

Permits and Restrictions

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Designations pCHR81
Depositors C Sasakawa
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 40.0000000000000000
Vector: pCHR81 (plasmid)
Construct size (kb): 40.0
Features: marker(s): kanR, tmpR
replicon: R388
vector useful for transposon mutagenesis
Restriction digests of the clone give the following sizes (kb): BamHI--32, 4.0, 1.8; PstI--36, 2.7, 1.8; HindIII--38, 3.8, 3.0.
Replication is temperature-sensitive.
Can be used as a suicide vector for the delivery of transposable elements.
Constructed by mutagenizing R388 to construct a temperature sensitive derivative and then transposing on Tn5.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C

Sasakawa C, Yoshikawa M. A series of Tn5 variants with various drug-resistance markers and suicide vector for transposon mutagenesis. Gene 56: 283-288, 1987. PubMed: 2824292

Shipped frozen