pCHR81 (ATCC® 37535)

Applications: vector useful for transposon mutagenesis  /  Depositors: C Sasakawa

Permits and Restrictions

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Designations pCHR81
Depositors C Sasakawa
Biosafety Level 1
Vector Information
Size (kb): 40.0000000000000000
Vector: pCHR81 (plasmid)
Marker(s):kanR,tmpR
Construct size (kb): 40.0
Features: marker(s): kanR, tmpR
replicon: R388
Applications
vector useful for transposon mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--32, 4.0, 1.8; PstI--36, 2.7, 1.8; HindIII--38, 3.8, 3.0.
Replication is temperature-sensitive.
Can be used as a suicide vector for the delivery of transposable elements.
Constructed by mutagenizing R388 to construct a temperature sensitive derivative and then transposing on Tn5.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C
References

Sasakawa C, Yoshikawa M. A series of Tn5 variants with various drug-resistance markers and suicide vector for transposon mutagenesis. Gene 56: 283-288, 1987. PubMed: 2824292

Shipped frozen
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