pBR327 (ATCC® 37516)

Depositors: EM Lederberg

Permits and Restrictions

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Designations pBR327
Depositors EM Lederberg
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.2730000019073490
Vector: pBR327 (plasmid)
Construct size (kb): 3.273000001907349
Features: marker(s): ampR, tetR
replicon: pMB1
A general purpose plasmid vector.
Derived from pBR322 by deleting sequences between nt 1430 (AvaI) and 2519, thus removing the bom or Mob site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Recomb. DNA Tech. Bull. 2: 1980..

Twigg AJ, Sherratt D. Trans-complementable copy-number mutants of plasmid ColE1. Nature 283: 216-218, 1980. PubMed: 7350544

Covarrubias L, et al. Construction and characaterization of new cloning vehicles V. mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328. Gene 13: 25-35, 1981. PubMed: 6263753

Balbas P, et al. Plasmid vector pBR322 and its special-purpose derivatives--a review. Gene 50: 3-40, 1986. PubMed: 3034735

Shipped freeze-dried