pSRW25 (ATCC® 37506)

Applications: expression vector  /  Depositors: CM Thomas

Permits and Restrictions

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Designations pSRW25
Depositors CM Thomas
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 12.6000003814697300
Vector: pSRW25 (plasmid)
Promoters: Promoter trp
Construction: pRK2501, EcoRI-SphI trpR from pSRW21
Construct size (kb): 12.60000038146973
Features: marker(s): kanR, tetR
promoter: trp
replicon: pMB1, RK2
expression vector
Plasmid can replicate in most gram-negative bacteria except Bacteroides.
Constructed to provide tight but inducible regulation from the trp promoter on a high copy number plasmid.
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C

Warne SR, et al. Use of a modified Escherichia coli trpR gene to obtain tight regulation of high-copy-number expression vectors. Gene 46: 103-112, 1986. PubMed: 3026921

Christopher M Thomas, personal communication

Shipped freeze-dried
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