lambda1059 (ATCC® 37474)

Applications: vector for constructing genomic librariesvector permitting positive selection for inserts  /  Depositors: S Brenner

Permits and Restrictions

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Designations lambda1059
Depositors S Brenner
Biosafety Level 1
Vector Information
Size (kb): 45.7999992370605500
Vector: lambda1059 (phage, lambda - replacement)
Promoters: Promoter none
Marker(s):ampR
Construct size (kb): 45.79999923706055
Features: insert detection: Spi+
marker(s): ampR
promoter: none
replicon: lambda
Applications
vector for constructing genomic libraries
vector permitting positive selection for inserts
Comments
The vector is Spi+; recombinants are Spi-.
Replacement vector useful for library construction which accepts inserts of 6-24 kb.
References

Karn J, et al. Novel bacteriophage lambda cloning vector. Proc. Natl. Acad. Sci. USA 77: 5172-5176, 1980. PubMed: 6254062

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet