pAL43 (ATCC® 37463)

Applications: vector for other uses  /  Depositors: AC Leonard

Permits and Restrictions

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Designations pAL43
Depositors AC Leonard
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.0000000000000000
Vector: pAL43 (plasmid)
Construction: oriC, pML21, Tn903
Construct size (kb): 7.0
Features: marker(s): kanR, neoR
replicon: oriC
vector for other uses
The plasmid minichromosome exhibits a moderate degree of instability and should be propagated in recA- strains grown in at least 50 mcg/ml kanamycin.
A plasmid which replicates from the resident chromosome origin of replication, oriC.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C

Leonard AC, Helmstetter CE. Cell cycle-specific replication of Escherichia coli minichromosomes. Proc. Natl. Acad. Sci. USA 83: 5101-5105, 1986. PubMed: 3523483

Leonard AC, et al. Involvement of DNA superhelicity of minichromosome maintenance in Escherichia coli. J. Bacteriol. 161: 687-695, 1985. PubMed: 2981821

Shipped freeze-dried