pAL43 (ATCC® 37463)

Applications: vector for other uses  /  Depositors: AC Leonard

Permits and Restrictions

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Designations pAL43
Depositors AC Leonard
Biosafety Level 1
Vector Information
Size (kb): 7.0000000000000000
Vector: pAL43 (plasmid)
Construction: oriC, pML21, Tn903
Marker(s):kanR,neoR
Construct size (kb): 7.0
Features: marker(s): kanR, neoR
replicon: oriC
Applications
vector for other uses
Comments
The plasmid minichromosome exhibits a moderate degree of instability and should be propagated in recA- strains grown in at least 50 mcg/ml kanamycin.
A plasmid which replicates from the resident chromosome origin of replication, oriC.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Leonard AC, Helmstetter CE. Cell cycle-specific replication of Escherichia coli minichromosomes. Proc. Natl. Acad. Sci. USA 83: 5101-5105, 1986. PubMed: 3523483

Leonard AC, et al. Involvement of DNA superhelicity of minichromosome maintenance in Escherichia coli. J. Bacteriol. 161: 687-695, 1985. PubMed: 2981821

Shipped freeze-dried
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