pUCTag (ATCC® 37460)

Applications: shuttle vector  /  Depositors: W Schaffner

Permits and Restrictions

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Designations pUCTag
Depositors W Schaffner
Biosafety Level 1
Vector Information
Size (kb): 5.9000000953674320
Vector: pUCTag (plasmid)
Construction: SV40, T-antigen, SV40 ori from pSVXB-p1, pUC18
Marker(s):ampR
Construct size (kb): 5.900000095367432
Features: marker(s): ampR
replicon: pMB1, SV40
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; HindIII/XbaI--2.9, 1.2, 1.1, 0.52; EcoRI--5.8.
A shuttle vector for cloning sequences which function in mammalian cells as enhancers.
The SV40 T antigen gene was cloned into pUC18.
The SV40 T-antigen gene and origin of replication from pSVXB-p1 was cloned as an AvaI/XbaI fragment into XbaI/HincII cut pUC18, after filling in the AvaI site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Tognoni A, et al. A novel expression selection approach allows precise mapping of the hepatitis B virus enhancer. Nucleic Acids Res. 13: 7457-7472, 1985. PubMed: 2997748

Shipped frozen
Shipping Information Distributed: freeze-dried
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