pUCTag (ATCC® 37460)

Applications: shuttle vector  /  Depositors: W Schaffner

Permits and Restrictions

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Designations pUCTag
Depositors W Schaffner
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.9000000953674320
Vector: pUCTag (plasmid)
Construction: SV40, T-antigen, SV40 ori from pSVXB-p1, pUC18
Marker(s):ampR
Construct size (kb): 5.900000095367432
Features: marker(s): ampR
replicon: pMB1, SV40
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; HindIII/XbaI--2.9, 1.2, 1.1, 0.52; EcoRI--5.8.
A shuttle vector for cloning sequences which function in mammalian cells as enhancers.
The SV40 T antigen gene was cloned into pUC18.
The SV40 T-antigen gene and origin of replication from pSVXB-p1 was cloned as an AvaI/XbaI fragment into XbaI/HincII cut pUC18, after filling in the AvaI site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Tognoni A, et al. A novel expression selection approach allows precise mapping of the hepatitis B virus enhancer. Nucleic Acids Res. 13: 7457-7472, 1985. PubMed: 2997748

Shipped frozen
Shipping Information Distributed: freeze-dried
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